Multilogic in Science (Journal for Applied Science)

ISSN : 2277 -7601

"An International Indexed & Peer Reviewed Quarterly Journal in Agriculture and Sciences"

web-based Editorial Management System

National Academy of Agricultural Sciences 2025

Article

DE NOVO ASSEMBLY AND ANNOTATION OF PERNA INDICA TRANSCRIPTOME USING NEXT GENERATION SEQUENCING TECHNOLOGY .

Sujitha Mary, Sandhya Sukumaran, Wilson Sebastian, A Gopalakrishnan

ICAR-Central Marine Fisheries Research Institute,

Ernakulam North P. O., Kochi - 682 018, Kerala, India .

sujithamary@gmail.com

Published On Oct 06, 2021

DOI :
https://doi.org/10.17605/OSF.IO/WJ89V

Full Article - PDF

Abstract
Indian coastline is bestowed with a rich diversity of molluscan species of which mussels belonging to the genus Perna (Family; Mytilidae)occupy a major position. Aquaculture importance of these species is globally recognized. They are extensively used as pollution indicatorsas they can accumulate contaminants at levels higher than that present in water. This property has made them valuable biomarkers ofenvironmental pollution. The scarcity in the genetic resources of this species is one of the major hindrances in its wide usage as abiomonitor. Here we sequenced the first transcriptome of Perna indica, collected from the southernmost part of Kerala coast, using NGSillumina HiSeq 2500 technology. The de novo assembly of Perna indica transcriptome generated 157203 contigs and 132641 non-redundantgenes with a minimum length of 201bp. The blast search and InterPro database scan resulted in assigning domains, ontology and pathwaymapping to these genes which helped in identifying genes involved in metabolism, immune related genes, proteins and domains associatedwith these genes. 84% of the transcripts which had atleast one blast-hit showed similarity to species from bivalves. 12420 transcripts withGO terms and 17835 transcripts with 395 KEGG pathways were identified in the assembled transcriptome of Perna indica.

Keywords
NGS, transcriptome, mussel, ontology, pathway mapping

Section
RESEARCH PAPER IN MARINE FISHERIES

How to Cite
Sujitha Mary, Sandhya Sukumaran, Wilson Sebastian, A Gopalakrishnan "DE NOVO ASSEMBLY AND ANNOTATION OF PERNA INDICA TRANSCRIPTOME USING NEXT GENERATIONSEQUENCING TECHNOLOGY"VOL. XII, ISSUE XXXX, Oct 2021, MULTILOGIC IN SCIENCE, ISSN 2277-7601, Page 186-190.

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